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1 Oz Wheatley’s Trichrome Stain for Identifing Parasites with Fine Tip Bottle

$ 15.81

Availability: 24 in stock

Description

This Wheatley’s Trichrome Stain is made by Hardy Diagnostics and is a
2021 fresh batch.  It's sold in 1 oz increments
in a
child-proof bottle
.  The Wheatley’s trichrome stain is used in the differentiation and rapid staining of intestinal parasites.  This kit includes a long pipet and precision-tip applicator bottle for transfering stain.
NOTE:  Orders of more than 1 ounce will be shipped in a single bottle with up to 20% discount in price.  Bottle size and color vary, depending on what I have on hand at the time your order is placed.
Parasitology Fecal Smears with Trichrome:
Artifacts and background material will stain green. Yeast, vegetable fibers, etc., also stain green. Red blood cells and bacteria usually stain red. Chromatin material and karyosomes of the nuclei will stain red to purple, as will helminth larvae and eggs. Cysts and organisms stain blue-green with a purple tinge. It is important to remember that there is a great deal of color variation in both the background and actual parasites.
Fecal Smears with Modified Trichrome Blue Stain:
The microsporidial spore wall should stain pink to red in color, with the interior of the spore being approximately 1-2um in size and clear or perhaps showing a horizontal or diagonal stripe representing the polar tube. The background will appear blue in color. Other bacteria, some yeast cells, and some debris will stain pink to red; the shapes and sizes of the components are useful in differentiating the spores from other structures. The results from this staining procedure should be reported only if the positive control smears.
Tissue Smears or Sections:
Muscle and cytoplasm stains red. Connective tissue stains green. Nuclei of the cells stains blue.
Trichrome Staining Tips
http://hardydiagnostics.com/wp-content/uploads/2016/05/Trichrome_Tips.pdf